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This profile of healthier skeletal muscle in both mdx tg and sgcd tg mice was also consistent with a reduction in immune cell infiltration as measured by cd45 and mac3 immunohistochemistry staining, as well as by a general reduction in inflammatory cytokine mrna levels in muscle from mdx or sgcd mice that contain the serpina3n transgene supplementary material, fig.
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Collectively, these data indicate that serpina3n overexpression potently inhibited increased serine protease activity after acute muscle injury, although this overexpression was of no observable harm to muscle.
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